This project aims to acquire and analyze physiological responses expressed by a participant during a simulated mammogram procedure to quantify the relative amount of stress and pain induced in this environment. Mammograms are routine procedures used to detect breast cancer and other tumors but are both mentally and physically taxing on patients. A multimodal approach is used to measure the participant’s physiological and psychological response during their interaction with Hologic’s 3Dimensions™ Mammography machine. The model considers metrics from Electrocardiogram (ECG), electromyography (EMG), galvanic skin response (GSR), and center of mass (COM) data sets as relative and reliable indicators of stress, pain, and focus. ECG measures the electrical activity of the heart, EMG measures the electrical activity in innervated muscles, and GSR measures change in skin conductance representative of sympathetic tone. The BIOPAC MP36R system is used to collect data during ECG and GSR measurements, the Delsys Trigno system is used to collect data from fourteen different muscles during the EMG measurements, and two AMTI Force Platforms are used to collect data during COM measurements. These various biophysical signals are simultaneously measured during four different paddle compressions utilizing both a traditional flat paddle and Hologic’s SmartCurve Paddle. Participants are presented with a questionnaire before and after the simulated procedure to determine their history with mammograms and acquire a self-reported measure of their experience. Analysis conducted on the resulting data will provide a list of indices from each respective signal allowing for a comprehensive comparison to be made between the two paddle designs as well as an overarching empirical-based conclusion on general mammography-related stress and pain.