This project aimed to continue developments an in vitro model for traumatic brain injury (TBI). Specifically, the group changed designs to the hammer and hammer components, designed a new release mechanism to allow for repeatable and consistent impact forces, and made progress on developing an ANSYS model to provide for a deeper understanding of the mechanics of impact. Additionally, the group researched and utilized a new cell dye for imaging the neural cells and tested the use of dissociated cells. Changes to the design of the apparatus were tested and verified using dry runs conducted with a sponge and wet runs conducted with neural tissue cultured in a scaffold. The different tests allowed for imaging of the impact through camera images and calculation of the force using force measurements. The new cell dye was verified by using hydrogen peroxide to induce apoptosis in 2-D cell cultures before testing with the 3-D. From the data collected, it was shown that the improvements made in this project were valid and made progress towards developing a more realistic in vitro testing device, though there is more work that must be completed in order to fully optimize the model. The requirement for this design is to develop an in vitro model to simulate traumatic brain injuries. The model should allow for further study of the effect of TBI on neurons in hopes of developing better preventative methods and treatments for TBIs. Improvement to the current model will include a more repeatable application of force on the cells, that is in the range similar to TBIs. The model should also be modified to allow for improved cell growth. Additionally, cell culture and imaging techniques will be refined allowing verification of axon network formation prior to the TBI and quantification of damage to cells post TBI.