Our senior design project focuses on developing a handheld bio-printing device that can be utilized in tissue defect repair and regeneration. The device consists of two syringes, one for the desired bio-ink and the other for the intended crosslinker, two servo motors, and a multichannel nozzle. Ultimately, this device would be used for in situ bio-printing, which is a novel technique of tissue repair where tissues are fabricated and/or repaired directly on the intended anatomical location of the defect, using the body itself as a bioreactor.